In this study, we found that the PD1 and PDL1 levels in spleens of HT-1080 tumor-bearing mice were significantly lower than those in the control group; meanwhile IFN-γ and TNF-α level in the serum of HT-1080 tumor-bearing mice were also significantly lower than those in the control group, which suggested that the antitumor mechanism of ZFSC on human fibrosarcoma could be attributed to inhibition of the PDL1/PD1 pathway, which could provide potential value to study the antitumor mechanism of ZFSC. Here, CD274 is linked to fibrosarcoma.