TWIST1 and neoplasm: EMT is a process by which epithelial cells lose their polarity and cell–cell adhesion and gain migratory and invasive properties, which has an important role in the initiation of tumor metastasis.2 Therefore, we sought to examine whether overexpression of ANG2 could affect the expressions of markers of EMT, such as vimentin, Snail, Twist and E-cadherin.12, 13 As shown in Figure 3b, the group of TCA8113 cells overexpressing ANG2 resulted in increased expression of vimentin, Snail and Twist, and reduced expression of E-cadherin, as compared with the group of TCA8113 mock cells.