IL10 and autosomal dominant polycystic kidney disease: For these studies, we first programmed RAW macrophages through incubation with ADPKD-CM or with control medium, after which: (1) the medium was collected over time for measurement of mouse IL-10 protein concentrations; and in parallel cultures, (2) the medium was washed out, and macrophages were incubated for a further 24 h in basal medium to produce CM that were assessed for pro-proliferative activity.