We incubated HEK-Blue cells expressing human TLR2, TLR3, TLR4, TLR5, TLR7, TLR8, TLR9, or the intracellular PRR protein NOD2 with 100 μg/ml DRibbles derived from the mycoplasma free lung cancer cell line UbiLT3. The gene discussed is TLR5; the disease is lung cancer.