We first performed a concentration-response experiment with HDM extract with a standard sensitization protocol (Supplemental Figure 3) to determine the amount of HDM extract (6.25 μg), which elicited minimal BHR, eosinophilia, and Ccl11 and Muc5ac mRNA (Supplemental Figure 8, B–E) in WT mice. This evidence concerns the gene CCL11 and Increased total eosinophil count.