To provide a direct evidence supporting the impact of histone modification on GAS5-AS1 expression in NSCLC cells, we performed additional experiments and found that specific knockdown of HDAC1 or HDAC3 with siRNA was able to significantly enhance the expression levels of GAS5-AS1 in H1299 (Supplementary Figure S2A) and PC-9 cells (Supplementary Figure S2B). The gene discussed is GAS5; the disease is non-small cell lung carcinoma.