In summary, we discovered that GAS5-AS1 was downregulated in NSCLC tumors and the reduced expression of GAS5-AS1 significantly associated with larger tumors (>3 cm), higher TNM stage, and lymph node metastasis, Further studies showed that ectopic expression of GAS5-AS1 inhibited NSCLC cell migration and invasion partially through repression of several key EMT markers. This evidence concerns the gene GAS5 and non-small cell lung carcinoma.