To validate the TAIT difference, we analyzed another set of surgical gastric carcinoma FFPE samples (Set-2) using MethyLight, and observed the same phenomenon: the average TAIT level in GFRA1 methylation-positive and -negative FFPE samples (n = 11 and 86) were 31.5 and 33.0, respectively (ΔCt = 1.5; P = 0.002, Figure 1B). Here, GFRA1 is linked to gastric carcinoma.