According to our results of genomic bisulfite sequencing, KDM4B, rather than DNMT1, DNMT3a, KDM4A, KDM4C, KDM5A or KDM6A, had a significant effect on the demethylation of the miR-615-5p promoter in HCC cells. Here, DNMT3A is linked to hepatocellular carcinoma.