We sought to define a threshold value for calling a tumor sample positive for PRAME expression (PRAME+), compare PRAME expression to the 1A/1B designation in Class 1 tumors, identify clinical and molecular factors associated with PRAME expression, evaluate the prognostic value of PRAME expression in Class 2 tumors, and determine whether PRAME expression in uveal melanoma is correlated with promoter hypomethylation. The gene discussed is PRAME; the disease is uveal melanoma.