The down-regulation of CFH transcription in AMD cybrids compared to age-matched Normal cybrids was supported by Western blot analyses demonstrating lower levels for the ~139 kDa CFH protein band (CFH isoform 1, representing the canonical sequence, p = 0.03) and also the ~50 kDa protein band (CFHL1 or CFH isoform 2, p = 0.001). This evidence concerns the gene CFHR1 and age-related macular degeneration.