CDC25A and brain neoplasm: To determine the role of Cdc25A-mediated PKM2 pS37 dephosphorylation in brain tumour development, we intracranially injected U87/EGFRvIII cells with or without depleted Cdc25A and reconstituted expression of rCdc25A or rCdc25A Y59F (Supplementary Fig. 6j) and U87/EGFRvIII cells with PKM2 depletion and reconstituted expression of rPKM2 WT or rPKM S37D (Supplementary Fig. 8a) into athymic nude mice.