To explore the effect of anti-TTx in favoring the responsiveness of the T cell compartment in breast cancer patients, the expansion of CD8+ memory T cells [7] that recognize HLA-A*02:01 restricted epitopes from HER2 and NY-ESO 1 was analyzed in PBMCs before and after anti-TTx in our patient stimulated with 2d-aDCs or 2d-stDCs as control APCs using two different in vitro systems (Additional file 2: Figure S2). This evidence concerns the gene CD8A and breast cancer.