LGALS4 and cyst: We further crossed 32 of the 69 genes to cell-type-specific Gal4s and found that 25 (25/32=78%) resulted in strong GSC loss with Nos-Gal4 (GSCs and early germline cysts) and various phenotypes with c587-Gal4 (CySCs and early cyst cells) (Supplementary Data 6 and Fig. 3).