FCS plus TGF-β did not change the expression of PVT1 up to 24 hours in ASMCs from healthy subjects (Fig 2, A), whereas at 24 hours, there was a significant reduction in the expression of PVT1 in ASMCs from patients with nonsevere asthma (P < .01 vs unstimulated control ASMCs). The gene discussed is PVT1; the disease is asthma.