Significant differences between SIRT3 positivity/high expression and cancer/non-cancer tissues were found in hepatocellular carcinoma (OR = 0.04, 95% CI = 0.01–0.16, P<0.0001) through subgroup analyses, suggested that expression of SIRT3 was downregulated in hepatocellular carcinoma tissues as compared with noncancerous tissues. This evidence concerns the gene SIRT3 and hepatocellular carcinoma.