In total effect analyses, data from 8 studies showed that there was no correlation between SIRT3 expression and cancer tissues, compared with non-cancer (adjacent and/or benign) tissues (OR = 0.34, 95% CI = 0.12–0.99, P = 0.05), and no significant correlation was found between SIRT3 expression and lymph node metastasis in total analyses. This evidence concerns the gene SIRT3 and cancer.