To determine whether unilateral stroke in the left mPFC was sufficient to elicit anxiety- or depression-like behavior or altered motor function, sham and stroke mice were compared using multiple behavioral tests.58 Beginning at 1 week after ET-1 injection, the mice were tested for anxiety- and depression-like behavior sequentially, with the least stressful anxiety tests first, then for locomotor function, at times shown in the timeline (Figure 4a). The gene discussed is EDN1; the disease is stroke disorder.