AKT1 and cancer: This result is consistent with previous work showing that a major substrate of Akt phosphorylation in cancer cells is the tuberous sclerosis complex protein TSC2 (Manning et al, 2002; Menon et al, 2014), which subsequently inhibits GTPase activating protein activity of TSC2, leading to the activation of the Rheb GTPase and hence mTORC1 (Manning et al, 2002; Menon et al, 2014).