IL10 and infection: The infection with Acanthamoeba trophozoites evoked a transient increase of IL-10 concentration by THP-1 cells that was significant only at 1.5 h postinfection (P value of <0.0001 by Student t test), whereas in PBMCs it was significant at both 1.5 and 3 h poststimulation (P value of <0.05 by Student t test) (Fig. 5A and B).