Collectively, the aim of the present study is 1) to determine whether there are differences for stress kinases related signaling pathways, ER stress, inflammation and AD related markers (i.e. APP, PS1 and PS2) in PBMCs from Chinese middle-aged men with varied body mass index (BMI); and 2) whether high glucose and fatty acids (FFAs) might be critical factors for affecting ER stress and AD markers under obese condition. The gene discussed is PSEN1; the disease is Alzheimer disease.