To optimize the operation procedures of MALDI-TOF MS, the gDNAs from PBMC of healthy individuals and the DNAs from two well-established lung adenocarcinoma cell lines in which H1975 harbors both EGFR L858R and T790M mutations and PC9 harbors Del19 mutation were subjected to test as reference materials. The gene discussed is EGFR; the disease is lung adenocarcinoma.