EGFR and lung carcinoma: Until now, many home-made and commercialized methods have been utilized for detecting the specific cancer associated gene mutations, such as EGFR mutations in lung cancer measured by Sanger sequencing, PCR-SSCP (single-strand conformation polymorphism), TaqMan PCR, Loop-hybrid mobility shift assay, cycleave PCR, and PCR-RFLP11, 12, 13, 14.