Cells from each source were rested for 24 hours in 10% FCS RPMI or stimulated with beads (bead/cell ratio, 1:1) and IL-2 (100 IU/mL) before FACS analysis to compare PTEN levels in T cells from a patient with PHTS versus a healthy control subject. This evidence concerns the gene PTEN and PTEN hamartoma tumor syndrome.