HMGB1 and acute respiratory distress syndrome: In order to outline the therapeutic importance of Fc‐mediated effector functions of h2G7 antibody in APAP‐ALI, we generated effector function‐deficient variants by modifying its CH2 domain either by site‐directed mutagenesis or by endoS treatment, which removes N‐linked glycosylation on the Fc part of antibodies.30, 31, 32 Importantly, none of the Fc‐modified variants of h2G7 affected the binding to HMGB1 (data not shown).