For that purpose, suspensions of cells from the HCC cell line Hep3B and LX-2 cells (LX-2-wt) stably expressing a short hairpin (sh) RNA directed against PAR2 (LX-2-shPAR2) and LX-2 cells with a non-target control shRNA, (LX-2-shCo) were injected into the right flank of SCID mice. This evidence concerns the gene F2RL1 and hepatocellular carcinoma.