F2RL1 and neoplasm: As demonstrated in Fig. 1f, the number of Ki67-positive cells (Hep3B and LX-2 cells) was apparently higher in tumours induced by coinjection of Hep3B cells with LX-2-wt cells (or LX-2-shCo) than with PAR2 deficient LX-2 cells (quantification by manual counting revealed approximately 2.5-fold higher number of Ki67-positive cells, data not shown), strongly suggesting that the reduced tumour growth mediated by silencing F2RL1 in LX-2 cells may depend on diminished cell proliferation in the tumour microenvironment.