Using univariate analysis, we discovered many significant prognostic factors for DFS or OS of HCC, including HBsAg, gender, AFP, AST, Neutrophil, CLIP score, TNM stages, PVTT, tumor size, tumor number, poor differentiation, intraoperative blood loss, NLR, PLR, ALRI, APRI and ANRI. The gene discussed is AFP; the disease is hepatocellular carcinoma.