The results revealed that preoperative ANRI (P < 0.001), HBsAg (P = 0.02), AFP (P < 0.001), AST (P < 0.001), Neutrophil (P = 0.018), CLIP score (P < 0.001), TNM (P = 0.001), PVTT (P < 0.001), tumor number (P < 0.001), tumor size (P < 0.001), tumor differentiation (P = 0.003), and intraoperative blood loss (P < 0.001), NLR (P < 0.001), APRI (P < 0.001), ALRI (P < 0.001), PLR (P = 0.025) were responsible for the DFS of HCC patients. The gene discussed is AFP; the disease is hepatocellular carcinoma.