Another unique approach to synthesizing an anti-tumor vaccine used multilayer self-assembly of components, with the T helper cell epitope as the core of the assembly, the immunostimulant γ-polyglutamic acid (γ-PGA) as the inner layer, and MUC1 glycopeptide as the outer layer, based on interactions between positive and negative charges [79]. This evidence concerns the gene MUC1 and neoplasm.