Pre-transplant MFC-MRD has been shown to be predictive for post-transplant outcome with high relapse rates of 60 to 70% after two or three years in MRD-positive patients as compared to only 8 to 21% in MRD-negative patients, respectively.16, 17, 18 The aim of this study was to evaluate the prognostic impact of pre-transplant NPM1 MRD levels determined by RT-qPCR in correlation to clinical characteristics and genetic abnormalities assessed at initial diagnosis in a cohort of adult AML patients receiving allogeneic HSCT. The gene discussed is NPM1; the disease is acute myeloid leukemia.