Conversely, treatment with the DNA methyltransferase inhibitor 5-aza-2′-deoxycytidine (5-Aza-dC) and the histone deacetylase inhibitor trichostatin A significantly upregulated ephrinB2 mRNA expression in glioma cell lines, supporting the role of promoter methylation in the control of ephrinB2 expression (Supplementary Fig. 1b,c). Here, EFNB2 is linked to glioma.