EFNB2 and neoplasm: Notably, the pro-invasive phenotype elicited by ephrinB2 deficiency was also seen in a collagen invasion assay, in which glioma spheroids lacking ephrinB2 invaded a collagen gel significantly more than WT glioma spheroids, suggesting a tumour cell intrinsic function of ephrinB2 (Supplementary Fig. 3e,f).