Subsequently, ephrinB2 knockout (KO) glioma cells were obtained by adenovirus-mediated expression of Cre recombinase fused to green fluorescent protein (GFP) (Ad-Cre-GFP), whereas expression of GFP alone (Ad-GFP) yielded control ephrinB2 wild-type (WT) glioma cells. Here, EFNB2 is linked to central nervous system cancer.