As a proof-of-concept, we applied our method to detect the most common TMPRSS2:ERG fusion transcript (TMPRSS2 exon1 to ERG exon 4) in cultured DuCap (TMPRSS2:ERG-positive) and LnCap (TMPRSS2:ERG-negative) human PCa cells6. Here, TMPRSS2 is linked to posterior cortical atrophy.