These genes were selected to represent different functions regulated by PPARα (lipogenesis: Mogat1; lipolysis: G0s2; fatty acid oxidation: Acot3; cholesterol metabolism: Hmgcr), As shown in Fig. 6a, Wy treatment led to induced hepatic Fgf21 expression and the 2-way ANOVA test indicated an interaction between NAFLD and the Wy response. The gene discussed is HMGCR; the disease is metabolic dysfunction-associated steatotic liver disease.