Metabolite profiling of Idh2R140Q mouse heart tissue reflected these observations, with significant increases in levels of glycolytic intermediates and decreases in steady-state levels of the TCA and anaplerotic metabolites, glutamate, malate and aspartate, consistent with observations in human cancer cells expressing IDH1/2 mutations (Reitman et al 2011). This evidence concerns the gene IDH1 and cancer.