To this end, CF mice were transiently transgenized with bIL-8-Luc.DNA construct, intratracheally challenged with hTNF-alpha (1 μg/mouse) and compared with WT mice (Fig. 1) Luc expression driven by bIL-8 activation at 4 h after TNF-alpha challenge (Fig. 1e) revealed a significant increase of BLI in CF mice in comparison with WT animals (3.1 and 1.2 FOI, p < 0.01) over baseline (untreated mice) (Fig. 1b, d, e). The gene discussed is TNF; the disease is cystic fibrosis.