We observed a distinct up regulation in GAS41 mRNA level both in HNGC2 and U87 cell lines compared to the normal cell (Fig 1A).Western blot analysis also showed similar up regulation of GAS41 in U87 and HNGC2 glioblastoma cell lines as compared to normal cell line demonstrating a clear retention of GAS41 expression at the translational level in both glioblastoma cell lines (Fig 1B). Further immunostaining experiments using GAS41 antibody also produced a similar pattern of expression in both HNGC2 and U87 cell lines (Fig 1C). This evidence concerns the gene YEATS4 and glioblastoma.