CNTN2 and glioblastoma: Thus we obscured miR-203 level by introducing antagomiR-203 in both HNGC2 and U87 cell lines (Fig 3D). Transfection with miR-203 in both the cell lines amplified GAS41 mRNA expression (Fig 3E). To facilitate GAS41- miR-203 regulationprototype, we over expressed GAS41 in glioblastoma cell lines by transfecting with pCMV-GAS41 construct which contains GAS41 coding region fused with C terminal FLAG into pCMV-Tag1 vector (S1 Fig) and evaluated miR-203 expression level.