In order to verify and visualize the effect we performed immunofluorescence staining studies in both the glioblastoma cell lines having over expressed GAS41 using anti GAS41 antibody (Fig 3C). Results clearly showed a reduced GAS41 signal upon miR-203 over expression, whereas no change was observed in glioblastoma cells transfected with empty vector. The gene discussed is YEATS4; the disease is glioblastoma.