Additional preclinical work is warranted to assess the impact of dual mTOR-PI3K and MEK-PI3K inhibition on a large panel of UC cell lines with a range of PI3K/AKT and MEK/ERK pathway genetic alterations to fully understand the context for optimal therapy of PI3K pathway-dependent bladder tumors. The gene discussed is PIK3CB; the disease is urinary bladder neoplasm.