We demonstrated that IL-6 is a Notch transcriptional target in MM cells by a dual luciferase assay in U266 cell line using an IL-6 reporter (pIL6-luc651) transactivated by plasmids carrying the active forms of NOTCH1 (ICN1) and NOTCH2 (ICN2). Here, NOTCH1 is linked to Miyoshi myopathy.