In the present study, we report the successful noninvasive detection of GBM tumors by PET imaging, using a radiolabeled MT1-MMP-specific mAb (LEM2/15) in mice xenografted with a glioma cell line expressing MT1-MMP or with patient-derived orthotopically implanted glioma tumor neurospheres. The gene discussed is MMP14; the disease is central nervous system cancer.