We confirmed our findings with RT-qPCR, identifying the expression of the genes GGA2, SPIB, CD200, STAG3, ADAM28, BCL2, VCAM1 and ICOSLG in B-cells isolated from an independent HPV(+) HNSCC tumor cohort (n=6) (Figure 6). The gene discussed is ICOSLG; the disease is head and neck squamous cell carcinoma.