In fact, intense GSDMB staining (Figure 2B, panel f) was found in HER2-positive tumours with clear GSDMB gene amplification (Figure 2A, panel d, more than 10 signals/nucleus); while undetectable or weak GSDMB staining was detected in normal breast tissue (Figure 2B, panel b) or lesions without ERBB2 or GSDMB amplification (GSDMB gene copy number of < 4 signals/nucleus, Figure 2A, panel c). Here, GSDMB is linked to neoplasm.