Our results also showed that GLS1 and GLUD1 were differentially expressed at the cytoplasm of prostate cancer cells (Figure 6A-F) and patients with tumors harboring high expression of GLS1 and GLUD1 had a significantly lower survival rate than those patients with tumors of low GLS1 and GLUD1 expression (Figure 6G and 6H). This evidence concerns the gene GLS and Familial prostate cancer.