Interestingly, previous reports have shown that whole-body KO of CRTC3 increases energy expenditure and protect the mutant mice from obesity and hepatic steatosis51, which appear to contradict our observation that CTRC3 knockdown prevents Ucp1 upregulation in Lkb1 KO adipocytes. This evidence concerns the gene CRTC3 and obesity due to melanocortin 4 receptor deficiency.