IDH1 and central nervous system cancer: To analyze the effects of IDH1R314C overexpression in eukaryotic cells we performed enzymatic assays with cell extracts from HEK293T cells, transiently overexpressing IDH1WT, IDH1R132H or IDH1R314C as well as lentivirally transduced LN229 and U251 glioma cells, stably expressing the different IDH1 mutants (Fig. 3A).