The immunostaining pattern was consistent with the flow cytometry results and indicated that LS180–Duke’s stage B, grade II, and SW 620–Duke’s stage C, grade IV, exhibited the highest levels of NEP compared to the other colon cancer cell lines examined (HT-29–Duke’s stage A, grade I and SW 948–Duke’s stage C, grade III). The gene discussed is MME; the disease is colonic neoplasm.