Our group has applied EB staining to mark BBB disruption in porcine NCC, demonstrating that treatment with praziquantel induces inflammation, cyst damage and BBB leakage at 2 and 5 days (48 and 120 h); we also have demonstrated that the BBB leakage is accompanied with expression of pro-inflammatory and immunoregulatory cytokines [5, 6]. The gene discussed is SLC12A3; the disease is cyst.