In line with the attenuated NF-κB activation and anti-apoptotic transcription, Olaparib or PJ-34 treatment triggered colon cancer cells to undergo spontaneous apoptosis, as conveyed by Annexin-V staining (Figure 7G and Figure 7—figure supplement 1C–D), which mirrors the impact of Sam68 knockdown on the survival of these cell lines (Figure 6G–H). This evidence concerns the gene NFKB1 and colonic neoplasm.