As shown in Supplementary Figure S8, our data showed that PAX3 re-expression increased phosphorylation of Akt and FOXO3a in U251 cells and phosphorylation of Erk in A375 cells, suggesting that PAX3 plays a tumor-promoting role in glioblastomas and melanomas probably through activating these signaling pathways. This evidence concerns the gene FOXO3 and melanoma.