BDNF and gastric cancer: Moreover, HTB135 cells secreted a higher mean amount of BDNF protein into conditioned media (CM) (0.531 ± 0.018 ng/ml) than nonmetastatic (0.060 ± 0.030 ng/ml), ascites (0.119 ± 0.021 ng/ml), or liver (0.049 ± 0.037 ng/ml) metastatic gastric cancer cells (Figure 1F, P <0.05), as measured by an enzyme-linked immunosorbent assay (ELISA); these results suggest that bone metastatic gastric cancer cells express elevated levels of BDNF/TrkB.