Since miR-1897-5p, miR-219-1-3p, and miR-376a# can directly or indirectly modulate several targets (shown in Additional file 1: Table S1), we decided to perform Western blot analysis of the following proteins linked to breast cancer: CCAAT/enhancer-binding protein beta (Cebpβ), caspase 3 (Casp3), insulin-like growth factor 1 receptor (Igf1r), protein kinase D1 (Pkd1), and transforming growth factor, beta receptor I (Tgfβr1). The gene discussed is TGFBR1; the disease is breast cancer.