At high affinity sites, Eguchi reported two important patterns of Scatchard analysis of anti-insulin IgG antibodies: (1) high (11.5 to 53.2) binding capacity (10−8 M) and low (0.04 to 0.21) affinity constant (108 M−1) in insulin autoimmune syndrome; and (2) low (0.12 to 1.1) binding capacity (10−8 M) and high (1.45 to 7.11) affinity constant (108 M−1) in patients with diabetes requiring insulin therapy [24]. The gene discussed is INS; the disease is diabetes mellitus.