A positive result was defined by circumferential membrane staining that is complete, intense, and within > 10 % of tumor cells via the IHC method and/or HER2 copy number ≥ 6 signal/cell or a signal ratio of HER2 gene to chromosome 17 ≥ 2.0 by the FISH method [8, 9]. Here, ERBB2 is linked to neoplasm.