To determine whether high levels of LC3B and Beclin-1 by Hcy treatment occur in a specific population of cells after cerebral ischemia, we co-stained for LC3B (or Beclin-1) and NeuN (neuron marker) (or glial fibrillary acidic protein (GFAP, astrocyte marker)). Here, BECN1 is linked to brain ischemia.