Therapy with PCAL not only reduced the growth of CRC but also augmented the tumoricidal effect of 5-FU, and they were significantly co-operated in inhibition of the colonic expression of well-known pro-oncogenic, angiogenesis- and metastasis inducing genes and molecules; such as Wnt/β-catenin signalling pathway, CDNK-1A, NF-kB, iNOS, Smads, HSP90, COX-2, Caspase-3, TGF-β1, and VEGF, that collectively have crucial roles in CRC development, promotion, invasion and metastasis. This evidence concerns the gene TGFB1 and colorectal carcinoma.