Additionally, the augmented anti-HCC activity of Ad-ΔB/TRAIL and Ad-ΔB/IL-12 combination therapy had also resulted in a more activation of the apoptotic-caspase-3 and-8 pathway, overproduction of interferon gamma (IFN-γ), high infiltration rate of natural killer cells and antigen presenting cells. The gene discussed is TNFSF10; the disease is hepatocellular carcinoma.